Extraction of high-quality RNA from mouse pancreatic tumors

Optimization of RNA Extraction from Rat Pancreatic Tissue

Background: Optimized RNA extraction from tissues and cell lines consists of four main stages regardless of the method of extraction: 1) homogenizing, 2) effective denaturation of proteins from RNA, 3) inactivation of ribonuclease, and 4) removal of any DNA, protein, and carbohydrate contamination. Isolation of undamaged intact RNA is challenging when the related tissue contains high levels of ...

optimization of rna extraction from rat pancreatic tissue

background: optimized rna extraction from tissues and cell lines consists of four main stages regardless of the method of extraction: 1) homogenizing, 2) effective denaturation of proteins from rna, 3) inactivation of ribonuclease, and 4) removal of any dna, protein, and carbohydrate contamination. isolation of undamaged intact rna is challenging when the related tissue contains high levels of ...

Extraction of high-quality RNA from pancreatic tissues for gene expression studies.

Extracting RNA from pancreatic tissue is notoriously challenging because of the organ's high RNase content. Standard methods using TriPure or TRIzol classically yield RNA of sufficient quality for routine gene expression analysis but not for microarray or deep sequencing analysis. Here we developed a simple method to extract high-quality RNA from mouse pancreas. Our method uses an RNase inhibit...

RNA isolation (Total RNA Extraction from High Polysaccharide Plants). Fatemeh Mehrpooyan*

DIE-RNA: A Reproducible Strategy for the Digestion of Normal and Injured Pancreas, Isolation of Pancreatic Cells from Genetically Engineered Mouse Models and Extraction of High Quality RNA

The isolation of ribonucleic acid (RNA) suitable for gene expression studies is challenging in the pancreas, due to its high ribonuclease activity. This is even more complicated during pancreatitis, a condition associated with inflammation and fibrosis. Our aim was to implement a time-effective and reproducible protocol to isolate high quality RNA from specific pancreatic cell subtypes, in norm...